JRC GMO-Amplicons | EURL GMFF

Record ID:	EF395436.1
Database:	nt	Go to original record
Description:	Tsuga diversifolia voucher A 1837-77 tRNA-Leu (trnL) gene, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tsuga diversifolia
Original size:	1470 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	470	1030	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaattcagg gaaccccggg atattttgaa
101   tgggtaatcc tgagccaaat ccggttcata gagacaatgg tttgtttctt
151   tcctaggata ggaaggggat aggtgcagag actcaatgga agctattcta
201   acgaatgaat ttcatttggg gtccaatact ctatttatag aacgctttat
251   ttacacctcg aaagtgggaa tgttgtataa aataaaacaa cactcgcgat
301   cagaacttga atcgtttcaa gcattttatt tgtaagagaa atgccagatt
351   tgagttgaag taatgatttg acattaagta atccaattat gaactttctc
401   tactctagat agggagttga atcagttttt ggaagaaatt attggacgag
451   gataaagata gagtccaatt ctacgtgtca atgtcaacaa caatgcaaat
501   tgcagtagga ggaaaatccg ttggctttat agaccgtgag ggttcaagtc
551   cctctatccc c

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details