JRC GMO-Amplicons | EURL GMFF

Record ID:	EF395426.1
Database:	nt	Go to original record
Description:	Tsuga chinensis voucher YU Havill 04-55 tRNA-Leu (trnL) gene, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tsuga chinensis
Original size:	1474 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	475	1046	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaattcagg gaaccccggg atattttgaa
101   tgggtaatcc tgagccaaat ccggttcata gagacaatgg tttctttcct
151   aggataggaa ggggataggt gcagagactc aatggaagct attctaacga
201   atgaatttca tttggggtcc aatactctat ttatagaacg ctttatttac
251   acctcgaaaa tttacacctc gaaagtggga atgttgtata aaataaaaca
301   acactcgcga tcagaatttg aatcgtttca agcattttat ttgtaagata
351   aatgccagat ttgagttgaa gtaatgattt gacattaagt aatccaatta
401   tgaactttct ctactctaga tagggagttg aatcagtttt tggaagaaat
451   tattggacga ggataaagat agagtccaat tctacgtgtc aatgtcaaca
501   acaatgcaaa ttgcagtagg aggaaaatcc gttggcttta tagaccgtga
551   gggttcaagt ccctctatcc cc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details