JRC GMO-Amplicons | EURL GMFF

Record ID:	MK141643.1
Database:	nt	Go to original record
Description:	Anthospermum whyteanum isolate cX96 tRNA-Thr (trnT(UGU)) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL(UAA)) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF(GAA)) gene, partial sequence; chloroplast.
Taxon:	Anthospermum whyteanum
Original size:	1745 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	786	1377	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcagaa
151   agtgaaaaaa aagggaaagg gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttggctg cggtagtcaa gaagtctttc catcaaaaat
251   tcataaacta taagtattaa ggatgaagtg aaggataaac gtatatacat
301   acgtagtcaa tagtatatta aatgagtaat gataacccga atgaatcttt
351   atttttctat aaaaaaataa agaattgttc ttaatagatt ccacgtttag
401   aaaagaatcg aatattcatt gatcaaatga ttcactccat agtctgatag
451   atcctttcac gaactgatta atcggacgag aataaagata gagtcccatt
501   ctacatgtca atgtcggcaa caatgaaatt tatagttaag aggaaaatcc
551   gtcgactttt aaaatcgtga gggttcaagt ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details