JRC GMO-Amplicons | EURL GMFF

Record ID:	MF287373.1
Database:	nt	Go to original record
Description:	Pogostemon cablin isolate Hainan chloroplast, complete genome.
Taxon:	Pogostemon cablin
Original size:	152462 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46853	47413	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaatta taattagatt gagccttggt
51    gtgggaaacc cactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttctc aaaacaaagg
151   ttcagaaaaa gaaatcaaag gataggtgca gagactcaac ggaagccgtt
201   ctaacaaatg gagttgacta cgttggtaga ggaatccttt ccacggaaac
251   ttcagaaagg ataaacctat ctattgaata ctatatcaaa tgattaatga
301   tggcccgaat ctgtattttt gaatatgaaa aatggaagaa ttggtgtgaa
351   ttgattctac attgaagaaa aaatagaata ttcattcatc aaatcattca
401   ctccatagtc cgataaatct tttttttaaa gaactgatta atcggacgag
451   aataaagata gagtcccatt ctacatgtca ataccggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

View other 11 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details