JRC GMO-Amplicons | EURL GMFF

Record ID:	MH115334.1
Database:	nt	Go to original record
Description:	Aristida adscensionis tRNA-Leu (trnL) gene, partial sequence; plastid.
Taxon:	Aristida adscensionis
Original size:	611 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	611	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc tcttttttga aaaacatgtg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga ggtaattacg ttgtgttggt agtggaattc tctctaaatt
251   agagaaagaa aaagaagggc tttatacatc taatacacac gtatagatac
301   tgacatagca aacgattaat cacaaaaccc atatcataat ataggttctt
351   ttttttttag aatgaaatta ggaatgatta tgaaataaaa aattcaaaat
401   tttggaatta ttgtgaatcc attccaatca aatattgagt aatcaaatcc
451   ttcaattcat tgttttcgag atcttttaaa aagtggatta atcggacgag
501   gataaagaga gagtcccatt ctacatgtca atactgacaa caatgaaatt
551   tctagtaaaa agaaaatccg tcgactttat aagtcgtgag ggttcaagtc
601   cctctatccc c

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details