JRC GMO-Amplicons | EURL GMFF

Record ID:	MH286315.1
Database:	nt	Go to original record
Description:	Phytolacca americana var. americana voucher M. J. Moore 342 (OC) chloroplast, complete genome.
Taxon:	Phytolacca americana var. americana
Original size:	155094 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47966	48582	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgaaaatttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa ctcctttttt caaaagaaaa aaataagggt
151   tcagaaagca agaaaaaaag gataggtgca gagactcaaa ggaagctgtt
201   ctaacaaatg gggttgactg tgctgtgttg ttataaaaat tcttccataa
251   aaattccaat caaaaaaaaa agggtgaagc atatacgtac tgaactatat
301   caaatgatta ataacaaccc gaatccttac ttttttttcg aatttttata
351   tatttatgaa atgaaaaaat ttatatgaaa aaaaaaaaaa atggaagaat
401   tttttggaat cgatttcaaa ttgaaagaag aatcaaatat tcattcatca
451   aataattcac tccatagtct gatagatttt ttgaagaact aattaatcgg
501   acgagaatga agatagagtc ccgttctaca tgtcaatact gacaacaatg
551   aaatttataa ttaagaagaa aatccgtcga ctttaaaaat cgtgagggtt
601   caagtccctc tatcccc

View other 6 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details