JRC GMO-Amplicons | EURL GMFF

Record ID:	MH921391.1
Database:	nt	Go to original record
Description:	Poa diaphora var. songarica voucher TARI<IRN>:Assadi & Mozaffarian 36867 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Poa diaphora var. songarica
Original size:	1745 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	819	1430	+	612 bp

Amplicon:

612 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc cagttaatta cgttgtgttg ttagtggaat tccttcgaaa
251   ttcgaaaaag aagggcttta tacatcacac gtatagatac tgagatagca
301   aacgattaat cacagaaccc atattataat attggttctt tattcttttt
351   tagaataaaa tttgaaatag aaatgatttt gaaataaaaa attcagaatt
401   tttttagaat tattgtgaat ccattccaat cgaatattta gtaatcaaat
451   ccttcaattc aaagttttga ggtctttaaa aaagtggatt aatcggacga
501   ggacaaagag agagtcccat tctacatgtc aatactgaca acaatgaaat
551   ttctagtaaa aggaaaatcc gtcgacttta taagtcgtga gggttcaagt
601   ccctctatcc cc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	612	593	2.76e-08

Match details