JRC GMO-Amplicons | EURL GMFF

Record ID:	MH921381.1
Database:	nt	Go to original record
Description:	Poa persica subsp. multiradiata voucher US:Soreng 9240 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Poa persica subsp. multiradiata
Original size:	1746 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	819	1436	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc cagttaatta cgttgtgttg ttagtggaat tccttcgaaa
251   ttcgaaaaag aagggcttta tacatctaat aaacacgtat agatactgag
301   atagcaaacg attaatcaca gaacccatat tataatattg gttctttatt
351   cttttttaga ataaaatttg aaatagaaat gattttgaaa taaaaaattc
401   agaatttttt tagaattatt gtgaatccat tccaatcgaa tatttagtaa
451   tcaaatcctt caattcaaag ttttgaggtc tttaaaaaag tggattaatc
501   ggacgaggac aaagagagag tcccattcta catgtcaata ctgacaacaa
551   tgaaatttct agtaaaagga aaatccgtcg actttataag tcgtgagggt
601   tcaagtccct ctatcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details