JRC GMO-Amplicons | EURL GMFF

Record ID:	MH181208.1
Database:	nt	Go to original record
Description:	Schizachyrium salzmannii isolate TK677 chloroplast, complete genome.
Taxon:	Schizachyrium salzmannii
Original size:	139775 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48001	48531	+	531 bp

Amplicon:

531 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataatata
251   ggttctttat ttgattttta gaatgaaatt aggaaggatt atgaaataga
301   aaattcataa tttttttaga attattgtga atctattcca atcgaatatt
351   gagtaatcaa atccttcaat tcattgtttt cgagatcttt taaaaataaa
401   aagtggatta atcggacgag gataaagaga gagtcccatt ctacatgtca
451   atactgacaa caatgaaatt tctagtaaaa ggaaaatccg tcgactttat
501   aagtcgtgag ggttcaagtc cctctatccc c

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	531	512	2.39e-08

Match details