JRC GMO-Amplicons | EURL GMFF

Record ID:	MH181191.1
Database:	nt	Go to original record
Description:	Andropogon gracilis isolate TK425 chloroplast, complete genome.
Taxon:	Andropogon gracilis
Original size:	139288 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48329	48878	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataatata
251   ggttctttac ccatattata atataggttc tttattttat ttttagaatg
301   aaattaggaa ggattatgaa atagaaaatt cataattttt ttagaattat
351   tgtgaatcta ttccaatcga atattgagta atcaaatcct tcaattcatt
401   gttttcgaga tcttttaaaa agtggattaa tcggacgagg ataaagagag
451   agtcccattc tacatgtcaa tactgacaac aatgaaattt ctagtaaaag
501   gaaaatccgt cgactttata agtcgtgagg gttcaagtcc ctctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details