JRC GMO-Amplicons | EURL GMFF

Record ID:	MH181184.1
Database:	nt	Go to original record
Description:	Andropogon abyssinicus isolate TK362 chloroplast, complete genome.
Taxon:	Andropogon abyssinicus
Original size:	138679 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47161	47692	+	532 bp

Amplicon:

532 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctttttttg aaaaaacaag cggttctcaa
151   actagaaccc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gaagtaataa cgattaatca cagaacccat attataatta
251   taatataggt tctttatttt atttttagaa tgcaatttgg aaggattatg
301   aaatagaaaa ttcataattt ttttagaatt attgtgaatc tattccaatc
351   gaatattgag taatcaaatc cttcaattca ttgttttcga gatcttttaa
401   aaagtggatt aatcggacga ggataaagag agagtcccat tctacatgtc
451   aatactgaca acaatgaaat ttctagtaaa aggaaaatcc gtcgacttta
501   taagtcgtga gggttcaagt ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	532	513	2.39e-08

Match details