JRC GMO-Amplicons | EURL GMFF

Record ID:	LN877388.1
Database:	nt	Go to original record
Description:	Dianthus gratianopolitanus chloroplast genome, complete sequence, isolate dg1370
Taxon:	Dianthus gratianopolitanus
Original size:	149511 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46608	47226	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aataaaaaaa
101   gggtaatcct gagccaactc cttttgtttc cttttttcaa aagaaaaaaa
151   ggataggtgc agagactcaa aggaagctgt tctaacaaat tgggttgact
201   gtgctgtgtt gttataagac ttcttccgtc taaattccca taaaaaaggg
251   ggggtaaaat cgacggagtg aaatgattaa tgacaacccg aatctgttct
301   gtattttttt atatattata tatatatata aatccatact ttattctata
351   tttagagtag agatctagaa gattaaatga aaaaatgcaa gaattcttgt
401   gaatccattc aaagttgaaa gcagaatcaa tcaagattta ttcattaaaa
451   tattcacact aactccatag tctgatagat cttgtgaaga acttattaat
501   cggatgagaa taaagataga gtcccgtact acatgtcaat actgacaaca
551   atgaaattta tagtaagagg aaaatccgtc gactttaaaa atcatgaggg
601   ttcaagtccc tctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details