JRC GMO-Amplicons | EURL GMFF

Record ID:	MH394429.1
Database:	nt	Go to original record
Description:	Hodgsonia macrocarpa chloroplast, partial genome.
Taxon:	Hodgsonia macrocarpa
Original size:	155055 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48834	49415	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aaagggtagg
151   tgcagagact caatggaaga tgttctaaca aatggagttg actacgttgc
201   gttagtaaag gaatgaatcc ttctatcgaa actccagaaa agatgaaaga
251   taaacgtatt acgtacggaa atactatttg attaatgaca acccgaatct
301   ctattttttt tttttataaa aaattatata taaaaatgaa cgaattgttc
351   tgaatcgatt ccaatatctc caagttgaaa aaagaatcga atattcattg
401   atcaaatcat ttactccatc atacatagtc tgatagatct tttgaagaac
451   taattaatca gatgcgaata gaataaagat agagtcccat tctacatgtc
501   aataccgaca aaaatgaaat ttctagtaag aggaaaatcc gtcgactttt
551   aaaatcgtga gggttcaagt ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details