JRC GMO-Amplicons | EURL GMFF

Record ID:	LS453397.1
Database:	nt	Go to original record
Description:	Iresine nitens trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence, isolate AC0795
Taxon:	Iresine nitens
Original size:	1037 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	110	665	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aaaatgggca atcctgagcc aactccttta ttaggaaaaa ataagaattc
151   agaaaacaag aataaaaaaa aaacaaagga taggtgcaga gactcaaagg
201   aagctgttct aacaaatgga gttgactgtg ctgtgttgtt ctaagaattc
251   tacttcaatc caatatgaat ataatatgaa atgaaaaaaa cctatatata
301   attattatta tgaatatgaa aaaatggaag aatttttacg aatcaaattc
351   aagttgaaat ttgaaaaaag aatcaaatat tcattcatca aatcattcac
401   tccatagtct gatagatctt ttgaagagct gattaatcgg acgagaataa
451   agatagagtc ccattctaca tgtcaatact gacaacaatg aaatttatag
501   taagaggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
551   atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details