JRC GMO-Amplicons | EURL GMFF

Record ID:	MG831870.1
Database:	nt	Go to original record
Description:	Adenocalymma ubatubense chloroplast, partial genome.
Taxon:	Adenocalymma ubatubense
Original size:	115404 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	34785	35351	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt cttaaaacaa aggttcagaa
151   aaggaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgccttggta gaggaatctt tccatcgaaa cttcagaaag
251   gatgaaggat aaacgtatct attgaatact atatcaatag attagtgata
301   gcccgaatct gtatctgtat tttttttata gtatatgaaa aatggaagaa
351   ttagtgtgaa ttgattacac attgaagaaa gaatcgaata ttcattcatc
401   aaatcattca ctccatagtc cgatagttct tttaaagaac tgattaagcg
451   gacgagaata aagatagagt cccattctac atgtcaatac cggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga cttttaaaat cgtgagggtt
551   caagtccctc tatcccc

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details