JRC GMO-Amplicons | EURL GMFF

Record ID:	KY999942.1
Database:	nt	Go to original record
Description:	Festuca sinensis isolate 7 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Festuca sinensis
Original size:	1044 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	619	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg aggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttctaaa
251   tttgagaaag aagggcttta tacacctaat aaacacgtat agatactgac
301   atagcaaacg attaatcacg gaacccatat tataatatag gttctttatt
351   cttttttaga atgaaatttg aaatagaaat gattatgaaa taaaaaattc
401   agaatttttt gtagaattat ttggaatcca ttccaattga atatttagta
451   atcaaatcct tcaattcaaa gttttgaggt ctttaaaaca gtggattaat
501   cggacgagga caaagagaga gtcccattct acatgtcaat actgacaaca
551   atgaaatttc tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg
601   ttcaagtccc tctatcccc

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details