JRC GMO-Amplicons | EURL GMFF

Record ID:	KT897736.1
Database:	nt	Go to original record
Description:	Colchicum triphyllum voucher E. Kaya_C0603 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Colchicum triphyllum
Original size:	1020 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	617	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttgatta gattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc tttatgttaa gaaaacaaag gataggtgca
151   gagactcaat ggaagctgtt ctaacaaatg gaattgacgt tgactacgtt
201   gcgttggtag ctggaatccc tctatcgaaa ttacagaaag gatgacccta
251   tatgcgtata tatacatact gacatatcaa acgattaatc acaactcgaa
301   tctggaatcc atattattaa ctataactat atattatagt tattattata
351   gatatatttt tatataaatt tatagattaa ttaagagtta ttataaatat
401   attccagtcg aagttgaagg aagaatcgaa tattcagtat tcagtgatca
451   aatcattcat tccggagtcg gatagatctt ttgaaaaaaa tgattaatcg
501   gacgagaata aagagagagt cctgttctac gtgtcaatac cgacagcaat
551   gaaatttata gtattaggaa aatccgtcga ctttagaaat cgtgagggtt
601   caagtccctc tatcccc

View other 10 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details