JRC GMO-Amplicons | EURL GMFF

Record ID:	LC206372.1
Database:	nt	Go to original record
Description:	Oxalis sp. AOKI2016 chloroplast gene for tRNA-Leu, trnL-trnF intergenic spacer, partial sequence, isolate: 341.
Taxon:	Oxalis sp. AOKI2016
Original size:	979 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	569	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctcttttccg aaaacaaaca aagttttcga
151   aagcgagaat aaaaaaggat aggtgcagag actcaacgga agctattcta
201   aaatttcaaa atagatttta ctgcgttgcg ttagtaaata aacccttccc
251   tcaaaattct agaaaggata aacctatata catacgtata cgtactgaaa
301   tactatttca aatgattaat gatgacccaa atctgtatta tttatcaaaa
351   aaatgaaaga tttgttgtga atcgattcca agttgaagaa agaatcgaat
401   gataattgat caaatcattc actccagtgt cggatagatc ttttgaaaaa
451   cggatagaat aaagatagag tcccattcta catgtcaata ttgacaacaa
501   tgaaatttat agtaagagga aaatccgtcg actttagaaa tcgtgagggt
551   tcaagtccct ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details