JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972894.1
Database:	nt	Go to original record
Description:	Vatica sarawakensis isolate 10-3487 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Vatica sarawakensis
Original size:	1825 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	825	1404	+	580 bp

Amplicon:

580 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaag
101   ggcaatcctg agccaaatcc tgtttaagtt caaaaagaaa taggataggt
151   gcagagactc aatggaagtt gttcgaacaa atggggttga ctgcttttgc
201   gttggtaaaa gaatacttct atcgaaactt cagcaaggat aaacatatag
251   aatacataat gaaaaaacta tttcaaaaaa gacgactggg acacctattc
301   ttttgatttc tattttttta taggttatag caaaaagaat tatatgaaaa
351   atcaaaagaa tccttgtgaa tcgatttcaa attgaagaaa gaatcaaata
401   gaatattcac taatcaaaca attcactcca tagtctgata aatcaatgga
451   ttaattggac gagaataaag atagagtccc attctacatg tcaatatcaa
501   taccgacaac aatgaaattt atagtaagag gaaaatccgt cgactttaaa
551   aatcgtgagg gttcaagtcc ctctatcccc

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	580	561	2.61e-08

Match details