JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972887.1
Database:	nt	Go to original record
Description:	Vatica micrantha isolate 01-0178 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Vatica micrantha
Original size:	1804 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	826	1385	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaag
101   ggcaatcctg agccaaatcc tgtttaagtt caaaaagaaa taggataggt
151   gcagagactc aatggaagtt gttcgaacaa atggggttga ctgcttttgc
201   gttggtaaaa gaatacttct tcagcaagga taaacatata gaatacataa
251   tgaaaaaact atttcaaaaa agacgactgc ttttgatttc tattttttta
301   taggttatag caaaaagaat tatatgaaaa atcaaaagaa tccttgtgaa
351   tcgatttcaa attgaagaaa gaatcaaata gaatattcac taatcaaaca
401   attcactcca tagtctgata aatcaatgga ttaattggac gagaataaag
451   atagagtccc attctacatg tcaatatcaa taccgacaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details