JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972762.1
Database:	nt	Go to original record
Description:	Pakaraimaea dipterocarpacea isolate 5169 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; plastid.
Taxon:	Pakaraimaea dipterocarpacea
Original size:	1881 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	858	1460	+	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gacttaagtg tattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaaa
101   tgggcaatcc tgagccaaat cctgttttac gagaataaaa aaaggttcaa
151   aaagcaagaa aaaaaatagg ataggtgcag agactcaatg gaagttgttc
201   gaacaaatgg ggttgactgc ttttgcgttg gtaaaagaat acttctatcg
251   aaactccagc aaggataaac atatagaata cgtaatgaaa aactatctca
301   aaaaagacga ctggggcacc tattcttttg atttgtttat aggttatagc
351   aaaaagaatt atatgcaaaa tcaaaagaaa agaatcgttg tgaatcgatt
401   ccaagttgaa gaatagaata ttcattaata aaaccattca ctccatagtc
451   tgataaatct tttgaaaaac ggattaatcg gacgagaata aagatagagt
501   cccgttctac atgtcaatat caataccgac aacaatgaaa tttatagtaa
551   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
601   ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	603	584	2.72e-08

Match details