JRC GMO-Amplicons | EURL GMFF

Record ID:	KY972733.1
Database:	nt	Go to original record
Description:	Dryobalanops aromatica isolate KASda2 trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; plastid.
Taxon:	Dryobalanops aromatica
Original size:	1997 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1031	1614	+	584 bp

Amplicon:

584 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aaaaaaaaag
101   ggcaatcctg agccaaatcc tgtttaaaaa agcgttcaaa aagaaatagg
151   ataggtgcag agactcaatg gaagttgttc gaacaaatgg ggttgaccgc
201   ttttgcgttg gtaaaagaat acttctatcg aaacttcagc aaggataaac
251   atatagaata cgtaatgaaa aattatctca aaaaaaacga ctgggacacc
301   tattcttttg atttctattt ttttataggt tatagcaaaa agaattatat
351   gaaaaatcaa aagaatcgtt gtgaatcgat ttcaaattga agaaagaatc
401   gaatagaata ttcattaata aaaccattca ctccatagtc tgataaatca
451   atgattaatt ggacgagaat aaagatagag tcccattcta catgtcaata
501   tcaataccga caacaatgaa atttatagta agaggaaaat ccgtcgactt
551   taaaaatcgt gagggttcaa gtccctctat cccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	584	565	2.63e-08

Match details