JRC GMO-Amplicons | EURL GMFF

Record ID:	MF035971.1
Database:	nt	Go to original record
Description:	Arundo donax isolate Teisher95 chloroplast, complete genome.
Taxon:	Arundo donax
Original size:	137172 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48419	49039	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaacaagtg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga ggtaattacg ttgtgttggt agtgaaactc cctctaaatt
251   agagaaagag gggctttgct ttatacatct aatacacaag tatagatact
301   gacatagcaa acgattaatc acagaaccca tatcataata taggttcttt
351   atttatttat tttttagaat gaaattagga atgattatga aatagaaaat
401   tcagaatttt tttagaatta ttgtgaatcc attccaatcg aatattgagt
451   aatcaaatcc ttcaattcat tgtttttgag atcttttaaa aagtggatta
501   atcggacgag gataaagaga gagtcccatt ctacatgtca atactgacaa
551   caatgaaatt tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag
601   ggttcaagtc cctctatccc c

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details