JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815454.1
Database:	nt	Go to original record
Description:	Empogona lanceolata voucher Bremer 4295 (S) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Empogona lanceolata
Original size:	1698 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	764	1326	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggctg cgttagtaga gaaatctttc catctaaaat tccgaaagga
251   taaagtgaag gataaacgta tatacgtatt gaatactata tcaaatgatt
301   aatgacgact cgactgaatc tgtattttta tataaaaacg gaagaattgg
351   tgtgaataga ttccacattg aagaaagaat cgaatattca ttgatcaaat
401   gattcactcc atagtctgat agatcttttc aagaatttat taatcggacg
451   agaataaaga tagagtcccg ttctacatgt caatgtcggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details