JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ815427.1
Database:	nt	Go to original record
Description:	Coffea macrocarpa voucher Razafimandimbison et al. 816 (S) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Coffea macrocarpa
Original size:	1686 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	750	1319	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggaaaggtt
151   cagaaagtga aaaaaggata ggtgcagaga ctcaacggaa gctgttctaa
201   caaatggagt tagctgcgtt agtagagaaa tctttccatc taaaattccg
251   aaaggataaa gtgaaggata aacgtatata cgtattgaat actatattaa
301   atgattaatg acgactcaac tgaatctgta ttttttatat aaaaatggaa
351   gaattggtgt gaatagattc cacattgaag aaagaatcga atattcattg
401   atcaaataat tcactccata gtctgataga tcttttcaag aattgattaa
451   tcggacgaga ataaagatag agtcccgttc tacatgtcaa tgtcggcaac
501   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcgtgagg
551   gttcaagtcc ctctatcccc

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details