JRC GMO-Amplicons | EURL GMFF

Record ID:	KF359905.1
Database:	nt	Go to original record
Description:	Oryza glumipatula chloroplast, complete genome.
Taxon:	Oryza glumipatula
Original size:	135224 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46742	47355	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   ggggcaatcc tgagccaaat ccgtgttttg agaaaacaag cggttctcga
151   actacaaccc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ctagcggaac tcccttctaa
251   attagagaaa gaagggcttt cgaaatctaa tacacacgta tagatactgg
301   catagcaaac gattaatcac agaactcata tcataatata tgttctttaa
351   ttctttttta gaatgaaaat aggaatgatt atgaaataga aaattcataa
401   tttttttaga attgttgtga atccattcca atcgaatatt gagtaatcaa
451   atccttcaat tcatagtttt caaatctttt taaaagtgga ttaatcggac
501   gaggataaag agagagtccc attctacatg tcaatactga caacaatgaa
551   atttctagta aaaggaaaat ccgtcgactt tctaagtcgt gagggttcaa
601   gtccctctat cccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details