JRC GMO-Amplicons | EURL GMFF

Record ID:	EF688821.1
Database:	nt	Go to original record
Description:	Heliotropium huascoense voucher 2909 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Heliotropium huascoense
Original size:	1012 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3	584	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa agaaaggttc
151   agaaagcaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggggtt gactgcattg gtcgaggaat ctttctatcg agacttcaga
251   aaggatgaag gataaacgta tatacatatg tattcaatac tctatcaaat
301   gaaatgatta atgacgaccc taatctgttc tgtttttttt atgaaaaaaa
351   aaggaagaat tggtgtgaat cgattccaca ttgaagttga agaaagaacc
401   gaatattcat tgatcaaatc actcactcca tagtctgata gttcttttga
451   agaactgatt aatcggacga gaataaagat agagtcccgt tctacatgtc
501   aataccggca acaatgaaat ttatagtaag aggaaaatcc gtcgacttta
551   aaaatcgtga gggttcaagt ccctctatcc cc

View other 14 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details