JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ787748.1
Database:	nt	Go to original record
Description:	Viola chaerophylloides voucher KWNU57081 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Viola chaerophylloides
Original size:	958 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	554	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acttactaag tggataactt tcaaattcag agaaaccctg gaataaaaaa
101   ggggcaatcc tgagccaaat ccgatttttt gaaatgaaaa aaaagtttat
151   atagacagaa taaataaaaa aggataggtg cagagactca atggaagctg
201   ttctaataaa tggagttgac tgtcttgcat tagtaaagta agggaacctt
251   tcgttaaaat tgcggattca aagtaaagga taaccctata aatacatata
301   cgtactgaaa gactatctca aataattaat gtaattatga aaaataaaat
351   aaaaatattg aatcgatttc aagttgaaga aaaaatcgat tgatcaaatc
401   attcactcca cagtctgata aataactaat taatcggacg agaataaaga
451   tagagtccca ttctacatgt taatattgac aacaaggaaa tttatagtaa
501   gaggaaaatc cgtcgacttt agaaatcgtg agggttcaag tccctctatc
551   ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details