Match details
Record ID: NRN_A18053
Database:
nrnl1
Go to original record
Description:
DNA for transforming monocotyledonous plants Sequence 1 from Patent WO9321335. Sequence 4 from Patent EP0955371. Sequence 4 from patent US 6002070. Sequence 4 from patent US 6074877. Sequence 4 from patent US 5641664. Sequence 1 from patent US 5679558. Sequence 4 from patent US 5712135.
Taxon:
unidentified
Original size:
4883 bp
Patents:
WO9209696 (lens , espacenet )
WO9321335 (lens , espacenet )
EP0955371 (lens , espacenet )
US6002070 (lens , espacenet )
US6074877 (lens , espacenet )
US5641664 (lens , espacenet )
US5679558 (lens , espacenet )
US5712135 (lens , espacenet )
View other 15 matches for this record
Method: QL-CON-00-015
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of the junction between the CaMV35S promoter and the bar gene (verified by the EURL GMFF in the context of Commission Decision 2006/578/EC).
Target: CaMV P-35S-bar
View other 358 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
1673
1808
+
136 bp
1 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
51 gaggacacgc tgaaatcacc agtctctctc tataaatcta tctctctctc
101 tataaccatg gacccagaac gacgcccggc cgacat
View other 148 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
21 bp
0
0
1
21
2.04e-09
primerB
100.0
22 bp
0
0
136
115
5.64e-10
probe
100.0
25 bp
0
0
22
46
1.44e-11
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
