Match details

Record ID: NRN_CQ876990
Database: nrnl1 Go to original record
Description: Sequence 36 from Patent WO2004081217. Sequence 36 from Patent WO2008049865.
Taxon: unidentified
Original size: 12776 bp
Patents: WO2004081217 (lens, espacenet) WO2008049865 (lens, espacenet)

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Method: QL-ELE-00-006 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (L 00.00-31, 1998).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 9602 9781 + 180 bp

Amplicon: 180 bp
1     gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
51    acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
101   tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
151   aaacaaaata tancgcgcaa actaggataa  

View other 15 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 8.54e-09
primerB 95.0 20 bp 1 0 180 161 1.10e-07


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.