JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_CS249198
Database:	nrnl1	Go to original record
Description:	Sequence 70 from Patent WO2005121347. Sequence 36 from Patent WO2006009659. Sequence 36 from patent US 7294756. Sequence 36 from patent US 7476778.
Taxon:	unidentified
Original size:	9616 bp
Patents:	WO2005121347 (lens, espacenet) WO2006009659 (lens, espacenet) US7294756 (lens, espacenet) US2006005280 (lens, espacenet) US7476778 (lens, espacenet) US2008172758 (lens, espacenet)

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Method:	QL-ELE-00-001	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:	CaMV P-35S

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	6073	6267	+	195 bp

Amplicon:

195 bp

1     gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat
51    gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
151   gtgacatctc cactgacgta agggatgacg cacaatccca ctatc

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	19 bp	0	0	1	19	3.10e-08
primerB	100.0	20 bp	0	0	195	176	9.28e-09

Match details