QL-ELE-00-001
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event Tomato Nema 282F | (Tomato)
|
| Genetic target: | CaMV P-35S |
| Description: | Qualitative PCR method for detection of CaMV 35S promoter (BVL, 1998) |
| Comments: | The fragment size generated by the CaMV P-35S-bar PCR depends on the assembly of the two sequence elements and is varying in the different GM plant events. Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Element specific |
| Analysis type: | Qualitative |
| Assay: | Endpoint PCR (Simplex) |
| Amplicon sequence: |
GCTCCTACAAATGCCATCATTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGA CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTC CACTGACGTAAGGGATGACGCACAATCCCACTATC Genbank ID: V00141 |
| Amplicon size: | 195 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | 35S-1 / | GCTCCTACAAATGCCATCA | 19 bp | CaMV P-35S |
| Primer reverse | 35S-2 / | GATAGTGGGATTGTGCGTCA | 20 bp | CaMV P-35S |
