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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CQ945863
Database: nrnl1 Go to original record
Description: Sequence 25 from Patent WO2004099447. Sequence 25 from patent US 7288643. Corn Event TC1507 and Methods for Detection Thereof. Sequence 25 from patent US 7417132. Sequence 25 from patent US 7435807. Sequence 25 from patent US 7449564. Sequence 25 from patent US 7514544. Sequence 25 from patent US 7989607.
Taxon: unidentified
Original size: 6186 bp
Patents: WO2004099447 (lens, espacenet) US7288643 (lens, espacenet) US2005039226 (lens, espacenet) JP2006525028 (lens, espacenet) US7417132 (lens, espacenet) US2008015346 (lens, espacenet) US7435807 (lens, espacenet) US7435807 (lens, espacenet) US7449564 (lens, espacenet) US2008132690 (lens, espacenet) US7514544 (lens, espacenet) US2008051569 (lens, espacenet) US7989607 (lens, espacenet)

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Method: QL-CON-00-011 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the Cauliflower Mosaic Virus 35S promoter and the pat gene
Target:CaMV P-35S-pat

View other 205 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 5224 5325 + 102 bp
Amplicon: 102 bp 
1     aagttcattt catttggaga ggacagggta cccggggatc caccatgtct
51    ccggagagga gaccagttga gattaggcca gctacagcag ctgatatggc
101   cg

View other 133 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 1.13e-11
primerB 100.0 21 bp 0 0 102 82 1.51e-09
probe 100.0 28 bp 0 0 51 78 2.64e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.