QL-CON-00-011
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event GS40/90pHoe6/Ac | ACS-BN010-4 (Oilseed Rape)
|
| Genetic target: | CaMV P-35S / pat |
| Description: | Qualitative PCR method for detection of the junction between the CaMV 35S promoter and the pat gene (BVL, 2012) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Construct specific |
| Analysis type: | Qualitative |
| Assay: | PCR (Simplex Real Time) |
| Amplicon sequence: |
AAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCCACCATGTCTCCGGAGAGGAGACCAGTTGAGATTAGGCCA GCTACAGCAGCTGATATGGCCG Genbank ID: FN550388 |
| Amplicon size: | 102 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | 35SP03.f / | AAGTTCATTTCATTTGGAGAGGACA | 25 bp | CaMV P-35S |
| Primer reverse | pat-7.r / | CGGCCATATCAGCTGCTGTAG | 21 bp | pat |
| Probe | GSS01.s / | FAM-CCGGAGAGGAGACCAGTTGAGATTAGGC-TAMRA | 28 bp |