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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS566036
Database: nrnl1 Go to original record
Description: Sequence 23 from Patent WO2006039376. Sequence 23 from patent US 7323556. CORN EVENT DAS-59122-7 AND METHODS FOR DETECTION THEREOF. Sequence 23 from patent US 7695914. Sequence 23 from patent US 7696341. Sequence 23 from patent US 7875429. Sequence 23 from patent US 7875430. Sequence 23 from patent US 7888023. Sequence 23 from patent US 7888495. Sequence 23 from patent US 7897342. Sequence 23 from patent US 7932033. Sequence 23 from patent US 7956246.
Taxon: unidentified
Original size: 11922 bp
Patents: WO2006039376 (lens, espacenet) US7323556 (lens, espacenet) US2006070139 (lens, espacenet) JP2008514233 (lens, espacenet) US7695914 (lens, espacenet) US7696341 (lens, espacenet) US7875429 (lens, espacenet) US7875430 (lens, espacenet) US7888023 (lens, espacenet) US7888495 (lens, espacenet) US7897342 (lens, espacenet) US7932033 (lens, espacenet) US7956246 (lens, espacenet)

View other 13 matches for this record

Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:CaMV P-35S

View other 10854 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 8665 8859 + 195 bp
Amplicon: 195 bp 
1     gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
51    gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
151   gtgatatctc cactgacgta agggatgacg cacaatccca ctatc

View other 8169 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 3.10e-08
primerB 100.0 20 bp 0 0 195 176 9.28e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.