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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS646798
Database: nrnl1 Go to original record
Description: Sequence 53 from Patent WO2007061845. Sequence 89 from Patent WO2007061742. DELTA 15 DESATURASES SUITABLE FOR ALTERING LEVELS OF POLYUNSATURATED FATTY ACIDS IN OLEAGINOUS PLANTS AND YEAST. DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS. Sequence 89 from patent US 7645604. Sequence 95 from patent US 7659120. Sequence 89 from patent US 8048653. Sequence 53 from patent US 8049062. Sequence 95 from patent US 8273957.
Taxon: unidentified
Original size: 12456 bp
Patents: WO2007061845 (lens, espacenet) WO2007061742 (lens, espacenet) JP2007515951 (lens, espacenet) JP2009517019 (lens, espacenet) US7645604 (lens, espacenet) US7659120 (lens, espacenet) US8048653 (lens, espacenet) US8049062 (lens, espacenet) US8273957 (lens, espacenet)

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Method: QL-ELE-00-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 6256 6337 + 82 bp
Amplicon: 82 bp 
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt

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Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.