QL-ELE-00-012
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View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event Bt11 | SYN-BT011-1 (Maize)
GMO event MON810 | MON-00810-6 (Maize) GMO event GA21 (G21) | MON-00021-9 (Maize) |
| Genetic target: | CaMV P-35S |
| Description: | Qualitative duplex PCR method for detection of CaMV 35S promoter; nopaline synthase (nos) terminator (target CaMV P-35S) (Waiblinger et al., 2008) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Element specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Multiplex) |
| Amplicon sequence: |
GCCTCTGCCGACAGTGGTNNCAAAGATGGACCCCCACCCACGNNNNNNNNNNNNNNNNNNGAAGACGTTCCAACCACGTC TT |
| Amplicon size: | 82 bp |
| Multiplex method: |
QL-ELE-00-012
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QL-ELE-00-013
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| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | 35S-FTM / | GCCTCTGCCGACAGTGGT | 18 bp | CaMV P-35S |
| Primer reverse | 35S-RTM / | AAGACGTGGTTGGAACGTCTTC | 22 bp | CaMV P-35S |
| Probe | 35S-TMP-FAM / | FAM-CAAAGATGGACCCCCACCCACG-BHQ1 | 22 bp |