Match details

Record ID: NRN_CQ945862
Database: nrnl1 Go to original record
Description: Sequence 24 from Patent WO2004099447. Sequence 24 from patent US 7288643. Corn Event TC1507 and Methods for Detection Thereof. Sequence 24 from patent US 7417132. Sequence 24 from patent US 7435807. Sequence 24 from patent US 7449564. Sequence 24 from patent US 7514544. Sequence 24 from patent US 7989607. Sequence 3 from Patent EP2513326.
Taxon: unidentified
Original size: 11361 bp
Patents: WO2004099447 (lens, espacenet) US7288643 (lens, espacenet) US2005039226 (lens, espacenet) JP2006525028 (lens, espacenet) US7417132 (lens, espacenet) US2008015346 (lens, espacenet) US7435807 (lens, espacenet) US7435807 (lens, espacenet) US7449564 (lens, espacenet) US2008132690 (lens, espacenet) US7514544 (lens, espacenet) US2008051569 (lens, espacenet) US7989607 (lens, espacenet) EP2513326 (lens, espacenet)

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Method: QL-CON-00-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the pat gene and the CaMV35S terminator (ISO/FDIS 21569:2005).
Target:pat-CaMV T-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 8559 8768 + 210 bp

Amplicon: 210 bp
1     atggtggatg gcatgatgtt ggtttttggc aaagggattt tgagttgcca
51    gctcctccaa ggccagttag gccagttacc cagatctgag tcgacctgca
101   ggcatgcccg ctgaaatcac cagtctctct ctacaaatct atctctctct
151   ataataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg
201   gtttcgctca  

View other 140 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 2.98e-09
primerB 100.0 23 bp 0 0 210 188 2.61e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.