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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX004880
Database: nrnl1 Go to original record
Description: Sequence 1 from Patent WO9910512. Sequence 1 from patent US 6140553. Sequence 1 from patent US 6372963. Sequence 2 from patent US 6483013. Sequence 1 from patent US 6323396. Improved Agrobacterium tumefacience-mediated transformation method of plants. JP 2000509612-A/1: Improved transformation method for plants.
Taxon: unidentified
Original size: 2345 bp
Patents: WO9910512 (lens, espacenet) US6140553 (lens, espacenet) US6372963 (lens, espacenet) US6372963 (lens, espacenet) US6483013 (lens, espacenet) US6483013 (lens, espacenet) US6323396 (lens, espacenet) US6323396 (lens, espacenet) JP2001514009 (lens, espacenet) JP2000509612 (lens, espacenet)

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Method: QL-CON-00-015 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the CaMV35S promoter and the bar gene (verified by the EURL GMFF in the context of Commission Decision 2006/578/EC).
Target:CaMV P-35S-bar

View other 358 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1330 1465 + 136 bp
Amplicon: 136 bp 
1     tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
51    gaggacacgc tgaaatcacc agtctctctc tataaatcta tctctctctc
101   tataaccatg gacccagaac gacgcccggc cgacat

View other 148 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 2.04e-09
primerB 100.0 22 bp 0 0 136 115 5.64e-10
probe 100.0 25 bp 0 0 22 46 1.44e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.