Match details

Record ID: NRN_FV533059
Database: nrnl1 Go to original record
Description: TRANSGENIC CROP PLANTS WITH IMPROVED STRESS TOLERANCE.
Taxon: unidentified
Original size: 293 bp
Patents: JP2009540830 (lens, espacenet)

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Method: QL-ELE-00-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (ISO/FDIS 21569:2005).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 2 49 243 + 195 bp

Amplicon: 195 bp
1     gcatctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat
51    tcctctgccg acagtggtcc caaagatgga cccccaccca ctaggagcat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
151   gtgatacttc cactgacgta agggatgacg cacaatccca ctatc 

View other 3 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 15 bp 0 0 5 19 5.18e-06
primerB 100.0 20 bp 0 0 195 176 9.28e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.