Match details

Record ID: NRN_AR260588
Database: nrnl1 Go to original record
Description: Sequence 16 from patent US 6489542. Sequence 16 from patent US 7700830. Sequence 16 from patent US 8030542. Sequence 16 from Patent EP2292759.
Taxon: unidentified
Original size: 8349 bp
Patents: US6489542 (lens, espacenet) US6489542 (lens, espacenet) US7700830 (lens, espacenet) US8030542 (lens, espacenet) EP2292759 (lens, espacenet)

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Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 216 411 + 196 bp

Amplicon: 196 bp
1     gcacctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat
51    gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
151   gtgatacttc cactgacgta agggaatgac gcacaatccc actatc 

View other 13 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 94.7 19 bp 1 0 1 19 1.45e-06
primerB 100.0 20 bp 0 0 196 177 9.33e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.