Match details
Record ID: HQ233646.1
Database:
emblreltgn
Go to original record
Description:
Gossypium hirsutum transgenic clone LLcotton25 phosphinothricin N-acetyltransferase gene, complete cds.
Taxon:
Gossypium hirsutum
Original size:
4644 bp
View other 33 matches for this record
Method: QL-CON-00-015
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of the junction between the CaMV35S promoter and the bar gene (verified by the EURL GMFF in the context of Commission Decision 2006/578/EC).
Target: CaMV P-35S-bar
View other 358 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
3030
3165
+
136 bp
1 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
51 gaggacacgc tgaaatcacc agtctctctc tataaatcta tctctctctc
101 tataaccatg gacccagaac gacgcccggc cgacat
View other 148 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
21 bp
0
0
1
21
2.04e-09
primerB
100.0
22 bp
0
0
136
115
5.64e-10
probe
100.0
25 bp
0
0
22
46
1.44e-11
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
