Match details
| Record ID: | MX927506.1 | |
|---|---|---|
| Database: | emblrelpat | Go to original record |
| Description: | Sequence 22 from patent US 11053544. | |
| Taxon: | unidentified | |
| Original size: | 2987 bp | |
| Patents: | US11053544 (lens, espacenet) | |
View other 4 matches for this record
| Method: | QL-ELE-00-025 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Qualitative duplex PCR method for detection of pat gene; bar gene (partim pat) (Debode et al., 2016). | |
| Target: | pat | |
View other 1741 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 0 | 0 | 53 | 160 | + | 108 bp |
| Amplicon: | 108 bp |
|---|
1 cgcggtttgt gatatcgtta accattacat tgagacgtct acagtgaact 51 ttaggacaga gccacaaaca ccacaagagt ggattgatga tctagagagg 101 ttgcaaga
View other 1557 matches with an identical amplicon
Primers and probe alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 22 bp | 0 | 0 | 1 | 22 | 4.75e-10 |
| primerB | 100.0 | 25 bp | 0 | 0 | 108 | 84 | 1.20e-11 |
| probe | 100.0 | 29 bp | 0 | 0 | 53 | 81 | 8.17e-14 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.