QL-ELE-00-025
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event Bt11 | SYN-BT011-1 (Maize)
|
| Genetic target: | pat |
| Description: | Qualitative duplex PCR method for detection of pat gene; bar gene (partim pat) (Debode et al., 2016) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Element specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Multiplex) |
| Amplicon sequence: |
CGCGGTTTGTGATATCGTTAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGACAGAGCCACAAACACCACAAGAGT GNNTTGATGATCTAGAGAGGTTGCAAGA |
| Amplicon size: | 108 bp |
| Multiplex method: |
QL-ELE-00-025
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QL-ELE-00-026
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| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | Pat-F / | CGCGGTTTGTGATATCGTTAAC | 22 bp | pat |
| Primer reverse | Pat-R / | TCTTGCAACCTCTCTAGATCATCAA | 25 bp | pat |
| Probe | Pat-P / | HEX-AGGACAGAGCCACAAACACCACAAGAGTG-BBQ | 29 bp |