Match details
| Record ID: | HZ040460.1 | |
|---|---|---|
| Database: | emblrelpat | Go to original record |
| Description: | JP 2015511118-A/194: PROCESSES FOR PRODUCING LIPIDS. | |
| Taxon: | synthetic construct | |
| Original size: | 16749 bp | |
| Patents: | JP2015511118 (lens, espacenet) | |
View other 44 matches for this record
| Method: | QL-ELE-00-018 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Qualitative PCR method for detection of nopaline synthase terminator (Barbau-Piednoir et al., 2014). | |
| Target: | T-nos | |
View other 14185 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 1 | 0 | 4850 | 4918 | - | 69 bp |
| Amplicon: | 69 bp |
|---|
1 GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT 51 AGCGCGCAAA CTAGGATAA
View other 14038 matches with an identical amplicon
Primers alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 28 bp | 0 | 0 | 237 | 264 | 4.42e-12 |
| primerA | 100.0 | 28 bp | 0 | 0 | 4918 | 4891 | 4.42e-12 |
| primerA | 100.0 | 28 bp | 0 | 0 | 15109 | 15136 | 4.42e-12 |
| primerA | 100.0 | 12 bp | 0 | 0 | 9411 | 9400 | 0.016 |
| primerA | 100.0 | 12 bp | 0 | 0 | 12073 | 12062 | 0.016 |
| primerB | 96.0 | 25 bp | 1 | 0 | 305 | 281 | 1.53e-09 |
| primerB | 96.0 | 25 bp | 1 | 0 | 4850 | 4874 | 1.53e-09 |
| primerB | 96.0 | 25 bp | 1 | 0 | 15177 | 15153 | 1.53e-09 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.