Match details
| Record ID: | HZ040457.1 | |
|---|---|---|
| Database: | emblrelpat | Go to original record |
| Description: | JP 2015511118-A/191: PROCESSES FOR PRODUCING LIPIDS. | |
| Taxon: | synthetic construct | |
| Original size: | 17146 bp | |
| Patents: | JP2015511118 (lens, espacenet) | |
View other 44 matches for this record
| Method: | QL-ELE-00-017 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Barbau-Piednoir et al., 2014). | |
| Target: | CaMV P-35S | |
View other 7323 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 1 | 0 | 16274 | 16348 | + | 75 bp |
| Amplicon: | 75 bp |
|---|
1 AAAGCAAGTG GATTGATGTG ATATCTCCAC TGACGTAAGG GATGACGCAC 51 AATCCCACTA TCCTTCGCAA GACCT
View other 809 matches with an identical amplicon
Primers alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 23 bp | 0 | 0 | 6297 | 6275 | 3.21e-09 |
| primerA | 100.0 | 23 bp | 0 | 0 | 13299 | 13321 | 3.21e-09 |
| primerA | 100.0 | 23 bp | 0 | 0 | 13626 | 13648 | 3.21e-09 |
| primerA | 100.0 | 23 bp | 0 | 0 | 15947 | 15969 | 3.21e-09 |
| primerA | 100.0 | 23 bp | 0 | 0 | 16274 | 16296 | 3.21e-09 |
| primerB | 100.0 | 20 bp | 0 | 0 | 6223 | 6242 | 1.56e-07 |
| primerB | 100.0 | 19 bp | 0 | 0 | 13699 | 13681 | 6.15e-07 |
| primerB | 100.0 | 19 bp | 0 | 0 | 16347 | 16329 | 6.15e-07 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.