Match details
| Record ID: | AR007527.1 | |
|---|---|---|
| Database: | emblrelpat | Go to original record |
| Description: | Sequence 17 from patent US 5750867. | |
| Taxon: | unidentified | |
| Original size: | 4808 bp | |
| Patents: | US5750867 (lens, espacenet) | |
View other 27 matches for this record
| Method: | QL-CON-00-015 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Qualitative PCR method for detection of the junction between the CaMV35S promoter and the bar gene (verified by the EURL GMFF in the context of Commission Decision 2006/578/EC). | |
| Target: | CaMV P-35S-bar | |
View other 358 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 0 | 0 | 3832 | 3967 | + | 136 bp |
| Amplicon: | 136 bp |
|---|
1 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 51 gaggacacgc tgaaatcacc agtctctctc tataaatcta tctctctctc 101 tataaccatg gacccagaac gacgcccggc cgacat
View other 148 matches with an identical amplicon
Primers and probe alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 21 bp | 0 | 0 | 1 | 21 | 2.04e-09 |
| primerB | 100.0 | 22 bp | 0 | 0 | 136 | 115 | 5.64e-10 |
| probe | 100.0 | 25 bp | 0 | 0 | 22 | 46 | 1.44e-11 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.