European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record HW810106.1 details
Record ID: HW810106.1
Database: emblrelpat Go to original record
Description: JP 2014534240-A/15: METHODS AND PRODUCTS FOR INCREASING FRATAXIN LEVELS AND USES THEREOF.
Taxon: Xanthomonas campestris
Original size: 5439 bp
Patents: JP2014534240 (lens, espacenet)

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Method QL-ELE-00-002 details
Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target: nptII

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Predicted amplification details:

Predicted amplification details
Mismatches Gaps From To Strand Amplicon size
0 0 3041 3249 - 209 bp

Amplicon size
Amplicon: 209 bp
1     CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG
51    CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
101   ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC
151   AGGATGATCA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG
201   CTCAAGGCG 

View other 103 matches with an identical amplicon


Primers alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 3249 3229 1.47e-08
primerB 100.0 21 bp 0 0 3041 3061 1.47e-08


Related publications:

  • Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
  • Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.