Match details
| Record ID: | HW671994.1 | |
|---|---|---|
| Database: | emblrelpat | Go to original record |
| Description: | JP 2013545454-A/17: Plant Expressiosn System. | |
| Taxon: | synthetic construct | |
| Original size: | 5743 bp | |
| Patents: | JP2013545454 (lens, espacenet) | |
View other 14 matches for this record
| Method: | QT-ELE-00-004 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter | |
| Target: | CaMV P-35S | |
View other 14461 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 0 | 0 | 936 | 1017 | + | 82 bp |
| Amplicon: | 82 bp |
|---|
1 GCCTCTGCCG ACAGTGGTCC CAAAGATGGA CCCCCACCCA CGAGGAGCAT 51 CGTGGAAAAA GAAGACGTTC CAACCACGTC TT
View other 24415 matches with an identical amplicon
Primers alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 18 bp | 0 | 0 | 609 | 626 | 7.39e-07 |
| primerA | 100.0 | 18 bp | 0 | 0 | 936 | 953 | 7.39e-07 |
| primerB | 100.0 | 22 bp | 0 | 0 | 690 | 669 | 4.24e-09 |
| primerB | 100.0 | 22 bp | 0 | 0 | 1017 | 996 | 4.24e-09 |
| probe | 100.0 | 22 bp | 0 | 0 | 629 | 650 | 4.24e-09 |
| probe | 100.0 | 22 bp | 0 | 0 | 956 | 977 | 4.24e-09 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.