Match details

Record ID:	JC529579.1
Database:	emblrelpat	Go to original record
Description:	Sequence 25 from Patent EP2730656.
Taxon:	synthetic construct
Original size:	8138 bp
Patents:	EP2730656 (lens, espacenet)

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Method:	QL-ELE-00-011	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of nopaline synthase terminator.
Target:	T-nos

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3877	3960	+	84 bp

Amplicon:

84 bp

1     catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
51    cgcaattata catttaatac gcgatagaaa acaa

View other 30799 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	25 bp	0	0	1	25	9.20e-12
primerB	100.0	25 bp	0	0	84	60	9.20e-12
probe	100.0	28 bp	0	0	28	55	2.28e-13

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.